Part:BBa_K108021

Fis

global DNA-binding transcriptional dual regulator of E.coli

Information contributed by City of London UK (2021)

Part information is collated here to help future users of the BioBrick registry.

Metadata:

Group: City of London UK 2021
Author: Lucas Ng
Summary: Added information collated from existing scientific studies

fis is an activator of ribosomal RNA transcription as well as other genes, as a key part in upstream up-regulation of rRNA promoters ^[1].

It binds to a recombinatorial enhancer helix-turn-helix sequence motif, facilitating hin-mediated DNA inversion ^[2] as well as binding to hundreds of transcriptionally active and inactive AT-rich sites ^[3] approximately half of which are located in non-coding DNA ^[4].

It prevents the initiation of oriC-mediated DNA replication ^[5].

References

↑ Ross, W., J. F. Thompson, J. T. Newlands, and R. L. Gourse. 1990. “E.coli Fis Protein Activates Ribosomal RNA Transcription in Vitro and in Vivo.” The EMBO Journal 9 (11): 3733–42. https://pubmed.ncbi.nlm.nih.gov/2209559.
↑ Safo, M. K., W. Z. Yang, L. Corselli, S. E. Cramton, H. S. Yuan, and R. C. Johnson. 1997. “The Transactivation Region of the Fis Protein That Controls Site-Specific DNA Inversion Contains Extended Mobile Beta-Hairpin Arms.” The EMBO Journal 16 (22): 6860–73. https://doi.org/10.1093/emboj/16.22.6860.
↑ Cho, B.-K., E. M. Knight, C. L. Barrett, and B. O. Palsson. 2008. “Genome-Wide Analysis of Fis Binding in Escherichia Coli Indicates a Causative Role for A-/AT-Tracts.” Genome Research 18 (6): 900–910. https://doi.org/10.1101/gr.070276.107.
↑ Grainger, David C., Douglas Hurd, Martin D. Goldberg, and Stephen J. W. Busby. 2006. “Association of Nucleoid Proteins with Coding and Non-Coding Segments of the Escherichia Coli Genome.” Nucleic Acids Research 34 (16): 4642–52. https://doi.org/10.1093/nar/gkl542.
↑ Wold, S. 1996. “The Escherichia Coli Fis Protein Prevents Initiation of DNA Replication from OriC in Vitro.” Nucleic Acids Research 24 (18): 3527–32. https://doi.org/10.1093/nar/24.18.3527.

[1] Ross, W., J. F. Thompson, J. T. Newlands, and R. L. Gourse. 1990. “E.coli Fis Protein Activates Ribosomal RNA Transcription in Vitro and in Vivo.” The EMBO Journal 9 (11): 3733–42. https://pubmed.ncbi.nlm.nih.gov/2209559.

[2] Safo, M. K., W. Z. Yang, L. Corselli, S. E. Cramton, H. S. Yuan, and R. C. Johnson. 1997. “The Transactivation Region of the Fis Protein That Controls Site-Specific DNA Inversion Contains Extended Mobile Beta-Hairpin Arms.” The EMBO Journal 16 (22): 6860–73. https://doi.org/10.1093/emboj/16.22.6860.

[3] Cho, B.-K., E. M. Knight, C. L. Barrett, and B. O. Palsson. 2008. “Genome-Wide Analysis of Fis Binding in Escherichia Coli Indicates a Causative Role for A-/AT-Tracts.” Genome Research 18 (6): 900–910. https://doi.org/10.1101/gr.070276.107.

[4] Grainger, David C., Douglas Hurd, Martin D. Goldberg, and Stephen J. W. Busby. 2006. “Association of Nucleoid Proteins with Coding and Non-Coding Segments of the Escherichia Coli Genome.” Nucleic Acids Research 34 (16): 4642–52. https://doi.org/10.1093/nar/gkl542.

[5] Wold, S. 1996. “The Escherichia Coli Fis Protein Prevents Initiation of DNA Replication from OriC in Vitro.” Nucleic Acids Research 24 (18): 3527–32. https://doi.org/10.1093/nar/24.18.3527.

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